Persistence of Mucosal Mast Cells and Eosinophils in Shigella-Infected Children
AUTOR(ES)
Raqib, Rubhana
FONTE
American Society for Microbiology
RESUMO
Cells of the innate immune system and their mediators were studied at the single-cell level in the rectums of pediatric and adult patients with Shigella infection to better understand why children are at higher risk for severe infection. Adult patients had increased infiltration of mucosal mast cells (MMC) at the acute stage (3 to 5 days after the onset of diarrhea) and eosinophils in early convalescence (14 to 16 days after onset). Increased expression of stem cell factor and prostaglandin H synthase-1 (PGHS-1) was associated with increased tryptase-Ki67-double-positive MMC in the acute stage and increased apoptosis of MMC, which led to a rapid decline in early convalescence. The eosinophils demonstrated increased expression of major basic protein (MBP), eotaxin, and CCR3, as well as increased necrotic death. The neutrophils showed enhanced α-defensin and lactoferrin expression in the acute phase. In contrast to adults, the pediatric patients demonstrated delayed accumulation of mast cells and eosinophils, while α-defensin expression persisted during convalescence. In contrast, neutrophil counts and lactoferrin expression were reduced in children compared to adults. The results suggest that children with shigellosis have a persistent activation of the innate immune response in the convalescent phase, indicating delayed elimination of Shigella antigens compared to adults.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=153256Documentos Relacionados
- Density of mucosal mast cells in the lamina propria of the colon and terminal ileum of children.
- Intestinal mucosal mast cells in normal and nematode-infected rat intestines are in intimate contact with peptidergic nerves.
- Lamina propria eosinophils and mast cells in ulcerative colitis: comparison between Asians and Caucasians.
- Human intestinal mucosal mast cells: evaluation of fixation and staining techniques.
- Effect of Treponema hyodysenteriae infection on mucosal mast cells and T cells in the murine cecum.