PHENANTHRENE AND ALKYL HOMOLOGOUS METABOLITES FORMATION AND IDENTIFICATION IN CRAB UCIDES CORDATUS / FORMAÇÃO E IDENTIFICAÇÃO DE METABOLITOS DE FENANTRENO E HOMÓLOGOS ALQUILADOS EM CARANGUEJO UCIDES CORDATUS

AUTOR(ES)
DATA DE PUBLICAÇÃO

2010

RESUMO

The main goal of this study was to evaluate the formation of phenanthrenes metabolites, parental and alkylated, in crabs Ucides cordatus urine. Laboratory experiments were carried out exposing organisms to phenanthrene (2 ug.kg-1 and 3 ug.kg-1), to the phenanthrene homologues alkylated, 1-methylphenanthrene and 2,6,9-trimethylphenanthrene (0,0125 ug.kg-1 and 0,025 ug.kg-1, respectively), to a Arabe oil and to a PAH contaminated sediment. The experiment maximum time was 96 h and at 0, 24, 48, 72 and 96 h were collected samples of: (1) urine to perform tests in order to evaluate the biological response about the formation of phenanthrene metabolites, (2) hemolymph to perform the micronucleus assay in order to verify cell mutations formation and (3) hepatopancreas to the determination of total PAH, especially this work¿s interest compounds. Methodologies were developed to metabolites measurements in crab¿s urine using kind of tools as the high performance liquid chromatography with fluorescence detection (HPLC/F) and high performance liquid chromatography with mass spectrometry detection (LC/MS). Were identified the formation of hydroxyphenanthrene (OHF) and other metabolites of phenanthrene, di- and tri-hydroxylated in urine samples injected with phenanthrene and trimethylphenanthrene. Both methods showed good sensitivity (LOQ = 0,001 and 0,01 ug.mL(-1), respectively) and good linearity (r2 = 0,9998 and 0,9997, respectively). There was no presence of mutated cells in the hemolymph slides in either group of bioassays. Analysis of PAH in hepatopancreas samples showed an increase in phenanthrene concentration in those groups of which the phenanthrene was the compound injected, and there was no concentrations change in methylphenanthrene on those groups injected with 1-methylphenanthrene and 2,6,9 - trimethylphenanthrene. The methods were showed to be effective in a preliminary assessment of phenanthrene and 2,6,9-trimethylphenanthrene metabolites formation. But the 1-methylphenanthrene and 2,6,9-trimethylphenanthrene low concentrations which the crabs were exposed, did not allow having a better evaluation of the data to this groups.

ASSUNTO(S)

fenantreno caranguejo alquilado

ACESSO AO ARTIGO

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