Phenolic acridine orange fluorescent stain for mycobacteria.
AUTOR(ES)
Smithwick, R W
RESUMO
A new fluorescence acid-fast staining method with acridine orange as the specific stain is presented. Only two reagents are required: the acridine orange-specific stain and a destaining-counterstaining reagent. Compared with auramine fluorescence acid-fast staining, there was less nonspecific staining of non-acid-fast debris which fluoresced a pale green contrasting color to provide a background in which to search for the red-to-orange fluorescing acid-fast bacilli. The results of the study indicate that the acridine orange method is superior to the auramine method in detecting acid-fast bacilli in specimen smears.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=228571Documentos Relacionados
- Acridine Orange Stain for Determining Intracellular Enteropathogens in HeLa Cells
- Evaluation of acridine orange stain for detection of microorganisms in blood cultures.
- Evaluation of acridine orange stain for detection of Trichomonas vaginalis in vaginal specimens.
- Microscopic observation of progressive immobilization of leishmania promastigotes in acridine orange stain.
- Evaluation of Acridine Orange Staining as a Replacement of Subcultures for BacT/ALERT-Positive, Gram Stain-Negative Blood Cultures
