Phenotypic Recognition of Pyocyanine Mutants in Pseudomonas aeruginosa

AUTOR(ES)

Carson, Melinda

RESUMO

Certain classes of pyocyanine mutants in Pseudomonas aeruginosa escape detection when screened in the presence of wild-type or other mutant cells. A technique is described for recognizing mutant phenotypes after cells are in individual agar wells. The procedure eliminates cross-feeding phenomena as well as the masking of mutant phenotypes by diffusing pyocyanine produced by nearby clones.

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