Phosphate-limited culture of Azotobacter vinelandii.

AUTOR(ES)

Tsai, J C

RESUMO

Batch cultures of Azotobacter vinelandii grown in phosphate-deficient media were compared with control cultures grown in phosphate-sufficient media. Phosphate limitation was assessed by total cell yield and by growth kinetics. Although cell protein, nucleic acids, and early growth rate were unaffected by phosphate deficiency, cell wall structure, oxygen uptake, and cell viability were significantly affected. Also, phosphate-limited cells contained much larger amounts of poly-beta-hydroxybutyric acid but lower adenylate nucleotide energy charge than did control cells. The ratio of adenosine 5'-triphosphate to adenosine 5'-diphosphate was much lower in phosphate-deficient cells. The data indicate a substrate saving choice of three metabolic pathways available to this organism under different growth conditions.

Documentos Relacionados

• Phosphate-limited continuous culture of Rhodotorula rubra: kinetics of transport, leakage, and growth.
• Continuous Culture of Rhodotorula rubra: Kinetics of Phosphate-Arsenate Uptake, Inhibition, and Phosphate-Limited Growth
• Phosphorylcholine stimulates capsule formation of phosphate-limited mucoid Pseudomonas aeruginosa.
• Kinetics of phosphate uptake, growth, and accumulation of cyclic diphosphoglycerate in a phosphate-limited continuous culture of Methanobacterium thermoautotrophicum.
• Recovery of competence in calcium-limited Azotobacter vinelandii.