Phosphate metabolites in lymphoid, Friend erythroleukemia, and HeLa cells observed by high-resolution 31P nuclear magnetic resonance.
AUTOR(ES)
Navon, G
RESUMO
High-resolution phosphorus-31 nuclear magnetic resonance spectra were observed for cell lines from mammalian sources: lymphoid cells, Friend erythroleukemia cells, and HeLa cells. Some phosphate metabolites were identified and their concentrations were found to vary among the different cell lines. ATP/ADP ratios could be measured in the living cells. The phospholipid metabolites phosphorylcholine and phosphorylethanolamine and their glyceryl esters were present in relatively high concentrations in the cells, but their ratios varied considerably. Also, a high concentration of an unidentified diphosphodiester compound was observed in the Friend erythroleukemia and HeLa cells. No appreciable pH gradient across the cell membrance was observed in these three mammalian cell lines, in contrast to the large pH gradient detected in microorganisms.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=411363Documentos Relacionados
- 31P nuclear magnetic resonance studies of HeLa cells
- High-resolution 31P nuclear magnetic resonance study of rat liver mitochondria.
- High-resolution 31P nuclear magnetic resonance studies of metabolism in aerobic Escherichia coli cells.
- High-resolution 31P nuclear magnetic resonance study of Chlamydia trachomatis: induction of ATPase activity in elementary bodies.
- High resolution 31P nuclear magnetic resonance studies of intact yeast cells.