Phosphorylation of Ribosome-Associated Protein by an Adenosine 3′:5′-Cyclic Monophosphate-Dependent Protein Kinase: Location of the Microsomal Receptor and Protein Kinase

AUTOR(ES)

Walton, Gordon M.

RESUMO

In the adrenal cortex, the adenosine 3′:5′-cyclic monophosphate (cAMP)-receptor protein and the cAMP-dependent protein kinase are located in both the cytosol and endoplasmic reticulum. The cAMP-dependent protein kinase from the cytosol catalyzes the phosphorylation of ribosome-associated protein. Ribosomes washed in 0.5 M NH4Cl retain the substrate of the protein kinase reaction, but are dependent on the NH4Cl extract for in vitro protein synthesis. Dissociation of the 80S ribosomes by 0.88 M KCl, however, releases the ribosome-associated (protein) substrate of the phosphorylation reaction.

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