Phosphorylation of the rev gene product of human immunodeficiency virus type 1.
AUTOR(ES)
Hauber, J
RESUMO
Replication of human immunodeficiency virus type 1 requires the functional expression in trans of the virally encoded rev gene product (previously called art/trs). Here we demonstrate that this protein can be metabolically labeled with 32Pi. The phosphate receptor in the rev protein is shown to be exclusively serine. Treatment of rev-expressing cells with phorbol ester, a specific activator of protein kinase C, led to significant but transient enhancement of the level of rev phosphorylation. These results indicate that the rev protein is posttranslationally modified in vivo and suggest that the level of this modification is subject to modulation by extracellular stimuli.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=253603Documentos Relacionados
- Purification and characterization of recombinant Rev protein of human immunodeficiency virus type 1.
- Regulation of human immunodeficiency virus env expression by the rev gene product.
- Different sites of interaction for Rev, Tev, and Rex proteins within the Rev-responsive element of human immunodeficiency virus type 1.
- Rev activates expression of the human immunodeficiency virus type 1 vif and vpr gene products.
- Gene inoculation generates immune responses against human immunodeficiency virus type 1.