Photoregulated expression of a pea rbcS gene in leaves of transgenic plants
AUTOR(ES)
Nagy, F.
RESUMO
A 2.4-kb pea genomic fragment, containing a member (rbcS-E9) of the multigene family encoding the small subunit (rbcS) of ribulose-1,5-bisphosphate carboxylase, was inserted into a non-oncogenic, Ti-plasmid vector and introduced into the genomes of Petunia hybrida (Mitchell) and Nicotiana tabacum (SR1) plants by in vitro transformation. Petunia and tobacco plants containing the introduced pea rbcS-E9 gene were regenerated from protoplasts. In these transgenic plants the rbcS-E9 gene is transcribed accurately using its own promoter and its expression is light-induced and organ-specific. A deletion mutant with 352 bp of 5'-upstream sequence still retains photoinducibility and leaf-specific expression. Clonal analysis of independent transgenic petunia plants revealed that chromosomal positions in the recipient plant genome affect the quantitative but not qualitative aspects of rbcS-E9 expression.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554623Documentos Relacionados
- Enhanced translation of a chloroplast-expressed RbcS gene restores small subunit levels and photosynthesis in nuclear RbcS antisense plants
- Expression dynamics of the tomato rbcS gene family during development.
- Inheritance, organization, and mapping of rbcS and cab multigene families in pea
- Several distinct types of sequence elements are required for efficient mRNA 3' end formation in a pea rbcS gene.
- Maize rbcS promoter activity depends on sequence elements not found in dicot rbcS promoters.