Physical and Genetic Map of the Obligate Intracellular Bacterium Coxiella burnetii
AUTOR(ES)
Willems, H.
FONTE
American Society for Microbiology
RESUMO
Pulsed-field gel electrophoresis and PCR techniques have been used to construct a NotI macrorestriction map of the obligate intracellular bacterium Coxiella burnetii Nine Mile. The size of the chromosome has been determined to be 2,103 kb comprising 29 NotI restriction fragments. The average resolution is 72.5 kb, or about 3.5% of the genome. Experimental data support the presence of a linear chromosome. Published genes were localized on the physical map by Southern hybridization. One gene, recognized as transposable element, was found to be present in at least nine sites evenly distributed over the whole chromosome. There is only one copy of a 16S rRNA gene. The putative oriC has been located on a 27.5-kb NotI fragment. Gene organization upstream the oriC is almost identical to that of Pseudomonas putida and Bacillus subtilis, whereas gene organization downstream the oriC seems to be unique among bacteria. The physical map will be helpful in investigations of the great heterogeneity in restriction fragment length polymorphism patterns of different isolates and the great variation in genome size. The genetic map will help to determine whether gene order in different isolates is conserved.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=107364Documentos Relacionados
- Biochemical stratagem for obligate parasitism of eukaryotic cells by Coxiella burnetii.
- Adenine Nucleotide Degradation by the Obligate Intracellular Bacterium Rickettsia typhi
- A physical map of the hyperthermophilic bacterium Aquifex pyrophilus chromosome.
- Biological Properties and Cell Tropism of Chp2, a Bacteriophage of the Obligate Intracellular Bacterium Chlamydophila abortus
- Genome size of the rickettsia Coxiella burnetii.