Physical characterization of ilv-lac fusions.
AUTOR(ES)
Leathers, T D
RESUMO
Electron microscopic heteroduplex analysis and comparative restriction digests have been used to characterize lambda p123(209), the complementary pair of phages used in the Casadaban technique of gene fusion. Derivatives of lambda 1(209) constructed to carry fusions of the lac genes to the control regions of the ilvC and ilvEDA operons were also analyzed. These physical maps have provided confirmation of the genetic models for these constructions and physical specifications important in interpreting the behavior of these ilv-lac fusions.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=216803Documentos Relacionados
- Characterization of fusions between the lac operon and the ilv gene cluster in Escherichia coli: ilvC-lac fusions.
- In vitro synthesis of beta-galactosidase with ilv-lac fusion deoxyribonucleic acid as template.
- Isolation and analysis of two Escherichia coli K-12 ilv attenuator deletion mutants with high-level constitutive expression of an ilv-lac fusion operon.
- Construction and characterization of Escherichia coli polA-lacZ gene fusions.
- Regulation of nitrogen fixation in Klebsiella pneumoniae: isolation and characterization of strains with nif-lac fusions.