Physiological, biochemical aspects and comparative proteomic during maturation, germination and seedling conversion of Ocotea catharinensis Mez. (Lauraceae). / Aspectos fisiológicos, bioquímicos e análise proteômica comparativa durante a maturação, germinação e conversão em plantas de embriões de Ocotea catharinensis Mez. (Lauraceae).

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The great similarity among the events of zygotic and somatic embryogenesis allows the establishment of parameters for accompaniment of these processes. In the present work it was studied the somatic embryogenesis in Ocotea catharinensis, in the maturation and germination phases, and the zygotic embryogenesis. The biochemical parameters and differential expression of proteins were evaluated: The treatment with ABA + PEG presented similar variations for zygotic embryos in the maturation stage. The germination was not observed for somatic embryos; however, it was verified that the previous dehydration promoted important biochemical alterations. With relation to the zygotic embryo, throughout the germination process, the synthesis of new proteins was not observed. The proteomic studies carried out throughout seed development, allowed the selection of polypeptides with differential expression. The results obtained open perspectives for the methodology optimization of the somatic embryogenesis, for species with recalcitrant seeds, like O. catharinensis.

ASSUNTO(S)

embriogênese somática plant physiology bioquímica de plantas plant biochemistry tissue culture plants somatic embryogenesis proteomics biotecnologia vegetal cultura de tecidos vegetais fisiologia vegetal plant biotechnology proteômica

ACESSO AO ARTIGO

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