Plaque Enhancement of Enteroviruses by Magnesium Chloride, Cysteine, and Pancreatin
AUTOR(ES)
Wallis, Craig
RESUMO
Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Fred Morales, Joycelyn Powell, and Joseph L. Melnick. Plaque enhancement of enteroviruses by magnesium chloride, cysteine, and pancreatin. J. Bacteriol. 91:1932–1935. 1966.—Plaque formation of 21 echoviruses (types 1–6, 9, 13, 15–19, 23–26, 29–32) and 8 coxsackieviruses (B1–6, A7, and A9) was enhanced by increased concentrations of MgCl2, l-cysteine, and pancreatin in agar overlay medium. In most cases, cationic and anionic polymers (diethylaminoethyl dextran, dextran sulfate, or protamine sulfate) were ineffective. All strains of poliovirus and group B coxsackieviruses were enhanced under agar by MgCl2. Five of the eight coxsackieviruses tested were also enhanced by cysteine or pancreatin. Certain enteroviruses, which have been difficult to assay by plaque method, can now be quantified effectively by incorporation of additives such as MgCl2, cysteine, or pancreatin into the overlay medium.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=316147Documentos Relacionados
- Sodium chloride, potassium chloride, and virulence in Listeria monocytogenes.
- MAGNESIUM METABOLISM IN HEALTH AND DISEASE. I. THE MAGNESIUM AND CALCIUM EXCRETION OF NORMAL INDIVIDUALS, ALSO THE EFFECTS OF MAGNESIUM, CHLORIDE, AND PHOSPHATE IONS
- Enhancement of Rhinovirus Plaque Formation in Human Heteroploid Cell Cultures by Magnesium and Calcium1
- Anaerobic Degradation of Cyanuric Acid, Cysteine, and Atrazine by a Facultative Anaerobic Bacterium
- Transport of sodium, chloride, and taurocholate by cultured rat hepatocytes
