Plasma rico em plaquetas no tratamento de tendinite em eqüinos: avaliação clínica, ultrasonográfica e histopatológica / Clinical, ultrasonographic and histopathological evaluation of tendinitis in horses treated with platelets-rich plasma

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Platelet-rich plasma (PRP) is an autologous and economic source of intensal growth factors, with great therapeutic potential. This work aimed to carry out clinical, ultrasonographic and histopathological evaluations of the effect of PRP on the treatment of induced tendonitis of the superficial digital flexor tendon (SDFT) of horses. Thus, six healthy geldings aged between eight and fifteen years ( x = 12 years) were evaluated. SDFT tendinitis was induced in both forelimbs, by means of intratendinous administration of 2.5 mg of collagenase (2.5 mg.mL-1), with such procedure being considered the start of the experimental phase. Twelve days after tendonitis induction, the animals were submitted to two treatments, with the lesion provoked in the right SDFT, (treated group TG), receiving 2.5 mL of PRP activated with calcium chloride at 10%, at concentrations ranging from 320.000 to 500.000 platelets.L-1; and the lesion in the left TFDS (control group - CG), receiving 2.5 mL of saline solution (NaCl 0.9%). Three days after the treatment started, the animals were submitted to controlled and progressive physical activity during 32 days. In the first 30 days after tendinitis induction, the animals were submitted to daily physical examination and were later monitored clinically twice a week until the end of the experiment. Ultrasonographic examinations were carried out before and after tendinitis induction (48 hours after and on experiment days 7, 12, 14, 21 28, 35 and 42), with tendon cross-sectional area (CSA), lesion cross-sectional area (L-CSA), L-CSA percentage, severity and ecchogenicity of the lesion and collagen fiber alignment being evaluated. On day 48, biopsy of the lesion site was conducted for the histopathological exam. Clinical evaluation after the treatments (from days 12 to 45) revealed, in function of the time, (P<0.05) reduced edema intensity, pain at palpation, local temperature increase, as well as degree of lameness. However, differences (P<0.05) between the groups were observed only for edema and pain at palpation, with less intensity in the TG. In the ultrasonographic examination, reduction (P<0.05) in the L-CSA and in lesion ecchogenicity, in function of time, was observed, but with difference (P<0.05) between groups only for the LCA, whose mean values were smaller in the TG. The histopathological evaluation revealed fibroblastic activity, neovascularization, lymphoplasmocitary infiltrate, hemorrhagic foci, and tissue disorganization in both groups. In the TG, the SDFT was more (P<0.05) organized with collagen fibers, and fibroblasts better displayed in the tendinous matrix. In addition, the qualitative analysis showed smaller (P<0.05) fibroblastic activity in the TG, which was not (P>0.05) confirmed by morphometry (quantitative analysis). The main conclusions of the study were that the PRP provides lower intensity of clinical variables (edema and pain at palpation), providing greater comfort and well being to the animal, as well as, promotes greater reduction in the area of the lesion and better tissue organization.

ASSUNTO(S)

tendon cavalos horses clinica veterinaria cicatrização tendão

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