Plasmid-borne Tn5 insertion mutation resulting in accumulation of gentisate from salicylate.
AUTOR(ES)
Monticello, D J
RESUMO
Plasmid-borne Tn5 insertion mutants of a Pseudomonas species which accumulated 2,5-dihydroxybenzoate (gentisate) following growth on 2-hydroxybenzoate (salicylate) were obtained from a pool of mutants that were unable to grow on naphthalene. One such mutant was characterized further. The ability of this mutant to oxidize gentisate was 100-fold less than the ability of a Nah+ Sal+ strain harboring the unmutagenized plasmid, although both strains oxidized and grew on salicylate. These bacteria were presumably able to metabolize salicylate via catechol, since they possessed an inducible, plasmid-encoded catechol 2,3-dioxygenase. Our results suggest that there is an alternate, plasmid-encoded route of salicylate degradation via gentisate and that some plasmid-associated relationship between this pathway and naphthalene oxidation exists.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=238441Documentos Relacionados
- Growth Rate-Dependent Accumulation of RNA from Plasmid-Borne rRNA Operons in Escherichia coli
- Transposition of Plasmid-Borne Tn10 Elements Does Not Exhibit Simple Length-Dependence
- Transfer of Plasmid-Borne Beta-Lactamase in Neisseria gonorrhoeae
- Novel type of plasmid-borne resistance to trimethoprim.
- Instability of a Plasmid-Borne Inverted Repeat in Saccharomyces Cerevisiae