Plasmid replication functions. IV. Promoters in the replication region of plasmid R6-5.

AUTOR(ES)

Slocombe, P M

RESUMO

Eight RNA polymerase binding sites have been shown to map within the EcoRI fragment E-2 (replication region RepA EcoRI fragment) of plasmid R6-5 and all but one have been shown to contain active promoters of transcription. Three of the identified promoters are located within a 2.7 kb region essential for controlled, autonomous plasmid replication and may be involved in the functional expression of the three R6-5 replication determinants that have thus far been identified, namely the origin of vegetative replication, oriV, the replication control gene, cop, and the determinant of an essential, positive-acting element, designated RepA.

Documentos Relacionados

• Identification of psiB genes of plasmids F and R6-5. Molecular basis for psiB enhanced expression in plasmid R6-5.
• Nucleotide sequence of the psiA (plasmid SOS inhibition) gene located on the leading region of plasmids F and R6-5.
• Regulation of DNA replication: "target" determinant of the replication control elements of plasmid R6-5 lies within a control element gene.
• Replication control mutations of plasmid R6-5 and their effects on interactions of the RNA-I control element with its target.
• Identification of a novel promoter in the replication control region of plasmid R6K.