Point mutational analysis of the Xenopus laevis 5S gene promoter.
AUTOR(ES)
Pieler, T
RESUMO
We have introduced C to T transitions into GC and CG base pairs of the Xenopus laevis somatic 5S gene coding region and its 5' flank in order to analyse their effects on transcription activity and regulation. These studies allow us to differentiate between the two promoter elements and their spacer, within the internal control region. Mutations within the 5' element which dissent from the corresponding tRNA consensus sequence reduce transcription activity substantially without significantly affecting transcription factor (TF) III A binding. Mutations in the spacer region have no pronounced effect on transcription. The 3' promoter element is found to extend to position 97, since mutations in this region interfere with transcription activity. This may be, at least partially, attributable to a reduced competition strength for TF III A.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=554426Documentos Relacionados
- Functional domains of the Xenopus laevis 5S gene promoter.
- Point mutation analysis of the Xenopus laevis RNA polymerase I core promoter.
- Torsional stress induces an S1 nuclease-hypersensitive site within the promoter of the Xenopus laevis oocyte-type 5S RNA gene.
- Linker scanner mutagenesis of the Xenopus laevis ribosomal gene promoter.
- DNA methylation patterns in the 5S DNAs of Xenopus laevis.