Poliovirus chimeras expressing sequences from the principal neutralization domain of human immunodeficiency virus type 1.
AUTOR(ES)
Dedieu, J F
RESUMO
Sequences from the principal neutralization domain of human immunodeficiency virus type 1 (HIV-1) strain LAI or RF have been expressed in antigenic site 1 of the capsid of the Sabin strain of poliovirus type 1. A number of the resulting chimeras were viable. Viable variants bearing mutations within the insertion site spontaneously arose from several nonviable chimeras. In general, these mutations result in a decrease in positive charge in the substituted antigenic site 1. Two of the chimeras were genetically stable and have been further characterized. Both chimeras were neutralized by various HIV-1 neutralizing antibodies. In rabbits, both chimeras produced high levels of antibodies which react with HIV-1 gp120/160 in immunoprecipitation and enzyme-linked immunosorbent assays. One of the chimeras (HIV-1LAI) produced a significant but weak HIV-1 neutralizing response.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=241080Documentos Relacionados
- The principal neutralization determinant of simian immunodeficiency virus differs from that of human immunodeficiency virus type 1.
- Principal neutralizing domain of the human immunodeficiency virus type 1 envelope protein.
- Mutations in the principal neutralization determinant of human immunodeficiency virus type 1 affect syncytium formation, virus infectivity, growth kinetics, and neutralization.
- Novel human endogenous sequences related to human immunodeficiency virus type 1.
- Autologous antibody response against the principal neutralizing domain of human immunodeficiency virus type 1 isolated from infected humans.