Polyma DNA: A Physical Map
AUTOR(ES)
Griffin, Beverly E.
RESUMO
The action of restriction enzymes on polyoma DNA was studied with uniformly 32P-labeled viral DNA obtained from either infected 3T6 or secondary mouse-embryo cells. Three restriction enzymes were used to construct a physical map of the polyoma genome. An enzyme from Hemophilus parainfluenzae, HpaII, cleaved polyma DNA into eight unique fragments (HpaII-1 to HpaII-8), ranging in size from 27.3 to 1.8% of the genome. An enzyme from Hemophilus influenzae, HinIII, gave two fragments (56 and 44%); and a third enzyme from Escherichia coli, EcoRI, cut at a single unique site. The physical map of the polyma genome was constructed from methods involving: (1) further digestion of the fragments produced by enzymes EcoRI and HinIII with HpaII, and (2) analysis of the products of partial digestion with HpaII.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=388389Documentos Relacionados
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