Polymer supported DNA synthesis using hydroxybenzotriazole activated phosphotriester intermediates.
AUTOR(ES)
Marugg, J E
RESUMO
Phosphorylation of N-acyl-5'-O-DMTr-d-nucleosides, or similarly protected DNA-dimers having a free 3'-OH group, with 2-chlorophenyl-0,0-bis(1-benzotriazolyl)phosphate affords reactive 3'-phosphotriester derivatives. The latter intermediates can be used, without further purification, for the synthesis of DNA-fragments on the controlled pore glass/long chain alkylamine support. Further, 2-cyano-1,1-dimethylethoxy dichlorophosphine proved to be very suitable for the preparation of 5'-phosphorylated DNA-fragments on the same type of solid support.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=320404Documentos Relacionados
- Synthesis of ppTppp via phosphotriester intermediates.
- Synthesis of 3'-phosphates of diribonucleoside monophosphates via phosphotriester intermediates.
- Polymer supported synthesis of oligonucleotides by a phosphotriester method
- A general method for the synthesis of 5'-monophosphates of DNA fragments via phosphotriester intermediates.
- New effective method for the synthesis of oligonucleotides via phosphotriester intermediates.
