Polymorphism in the Plasmodium vivax msp 3: gene in field samples from Tierralta, Colombia
AUTOR(ES)
Cristiano, Fabio Aníbal, Pérez, Manuel Alberto, Nicholls, Rubén Santiago, Guerra, Angela Patricia
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2008-08
RESUMO
We evaluated the Plasmodium vivax polymorphism by studying the Pvmsp-3 gene's polymorphic region by PCR-RFLP in 55 samples from patients living in Tierralta, Colombia. Three different sizes of the Pvmsp-3 gene were found, type A (1,900 bp), type B (1,500 bp) and type C (1,100 bp); most of the samples were type A (96.4 %). The Pvmsp-3 gene exhibited high polymorphism. Seven restriction patterns were found when using Alu I, and nine were found with Hha I; 12 different alleles were obtained when these patterns were combined. The findings suggest that this gene could be used in Colombia as a molecular epidemiologic marker for genotyping P. vivax.
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