Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene

AUTOR(ES)

Bhushan, Bharat, Patra, Biswa Nath, Das, Pranab Jyoti, Dutt, Triveni, Kumar, Pushpendra, Sharma, Arjava, Umang, , Dandapat, Satyabarta, Ahlawat, Shiv Pal Singh

FONTE

Genetics and Molecular Biology

DATA DE PUBLICAÇÃO

2007

RESUMO

The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI- and HinfI- RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is highly polymorphic in cattle.

Documentos Relacionados

• A primate species with limited major histocompatibility complex class I polymorphism.
• Association of the bovine major histocompatibility complex (BoLA) BoLA-DRB3 gene with fat and protein production and somatic cell score in Brazilian Gyr dairy cattle (Bos indicus)
• Functional expression of a heterologous major histocompatibility complex class I gene in transgenic mice.
• Evidence for extensive polymorphism of class I genes in the rat major histocompatibility complex (RT1).
• Identification of a shark sequence resembling the major histocompatibility complex class I alpha 3 domain.