Polynucleotide-histone H1 complexes as probes for blot hybridization.

AUTOR(ES)

Renz, M

RESUMO

Biotin and/or 125I-labelled histone H1 proteins (21 kd) have been covalently bound to single-stranded DNA. Complexes of equal masses of DNA and modified histone H1 (approximately 1 histone H1 molecule per 70 nucleotides) were used as probes for blot hybridization experiments and found to have hybridization characteristics very similar (or identical) to radiolabelled DNA probes.

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