Polyphosphoinositides Are Present in Plasma Membranes Isolated from Fusogenic Carrot Cells 1
AUTOR(ES)
Wheeler, Jeffery J.
RESUMO
Fusogenic carrot cells grown in suspension culture were labeled 12 hours with myo-[2-3H]inositol. Plasma membranes were isolated from the prelabeled fusogenic carrot cells by both aqueous polymer two-phase partitioning and Renografin density gradients. With both methods, the plasma membrane-enriched fractions, as identified by marker enzymes, were enriched in [3H]inositol-labeled phosphatidylinositol monophosphate (PIP) and phosphatidylinositol bisphosphate (PIP2). An additional [3H]inositol-labeled lipid, lysophosphatidylinositol monophosphate, which migrated between PIP and PIP2 on thin layer plates, was found primarily in the plasma membrane-rich fraction of the fusogenic cells. This was in contrast to lysophosphatidylinositol which is found primarily in the lower phase, microsomal/mitochrondrial-rich fraction.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1054266Documentos Relacionados
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