Polyribosome Isolation in the Presence of Diethyl Pyrocarbonate 1

AUTOR(ES)

Weeks, Donald P.

RESUMO

Isolation of polyribosomes from wheat embryos and corn root tips in the presence of diethyl pyrocarbonate showed this reagent to have a protective effect on polyribosome structure. In addition, the use of diethyl pyrocarbonate allowed initial homogenization to be performed under less stringent conditions than those normally employed for polyribosome isolation. The use of the reagent is however limited, in that it is deleterious to in vitro ribosomal amino acid incorporation.

Documentos Relacionados

• The Effects of Diethyl Pyrocarbonate on the Stability and Activity of Plant Polyribosomes 1
• Cyclization of uridine monophosphate by diethyl pyrocarbonate.
• Diethyl pyrocarbonate: a chemical probe for DNA cruciforms.
• Reaction of Diethyl Pyrocarbonate with Nucleic Acid Components, I. Adenine*
• Histidine modification with diethyl pyrocarbonate shows heterogeneity of benzodiazepine receptors.