Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site.
AUTOR(ES)
Peña, C E
RESUMO
Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNAGly gene at attB. We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=178380Documentos Relacionados
- Integration specificities of two lambdoid phages (21 and e14) that insert at the same attB site.
- Mycobacteriophage L5 integrase-mediated site-specific integration in vitro.
- A novel host factor for integration of mycobacteriophage L5
- The cohesive ends of mycobacteriophage L5 DNA.
- Site-specific integration of the temperate bacteriophage phi adh into the Lactobacillus gasseri chromosome and molecular characterization of the phage (attP) and bacterial (attB) attachment sites.
