Possible Mechanism of Miltefosine-Mediated Death of Leishmania donovani†

AUTOR(ES)

Verma, Navin K.

FONTE

American Society for Microbiology

RESUMO

Miltefosine causes leishmanial death, but the possible mechanism(s) of action is not known. The mode of action of miltefosine was investigated in vitro in Leishmania donovani promastigotes as well as in extra- and intracellular amastigotes. Here, we demonstrate that miltefosine induces apoptosis-like death in L. donovani based on observed phenomena such as nuclear DNA condensation, DNA fragmentation with accompanying ladder formation, and in situ labeling of DNA fragments by the terminal deoxyribonucleotidyltransferase-mediated dUTP-biotin nick end labeling method. Understanding of miltefosine-mediated death will facilitate the design of new therapeutic strategies against Leishmania parasites.

Documentos Relacionados

• Miltefosine Induces Apoptosis-Like Death in Leishmania donovani Promastigotes
• Leishmania donovani Resistance to Miltefosine Involves a Defective Inward Translocation of the Drug
• Miltefosine induces programmed cell death in Leishmania amazonensis promastigotes
• Oxidant-mediated damage of Leishmania donovani promastigotes.
• Mechanism of Amphotericin B Resistance in Leishmania donovani Promastigotes