Post-transcriptional regulator (rex) of HTLV-1 initiates expression of viral structural proteins but suppresses expression of regulatory proteins.
AUTOR(ES)
Hidaka, M
RESUMO
Gene expression of human T-cell leukemia virus type 1 (HTLV-1) is regulated by two trans-acting factors encoded by the pX region, p40tax and p27tax.p40tax is a transcriptional activator and p27rex is a post-transcriptional regulator. Using full-length viral DNA, we studied the regulatory effects of rex on HTLV-1 gene expression. p27rex is required for expression of both gag and env proteins, increasing the level of their mRNAs. The effect was dependent on the dose of p27rex expression plasmid. In parallel, increased doses of p27rex suppressed the expression of fully spliced pX mRNA, which encodes the regulatory proteins. These two effects of p27rex operated at the post-transcriptional level and were independent of transcriptional regulation. Lowering the level of pX mRNA down-regulates transcription of the proviral genome. These observations demonstrate that rex is a positive post-transcriptional regulator for gag, pol and env protein expression, and acts at the same time as an indirect negative regulator of viral transcription.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=454349Documentos Relacionados
- Visna virus encodes a post-transcriptional regulator of viral structural gene expression
- Visna virus encodes a post-transcriptional regulator of viral structural gene expression.
- Multiple post-transcriptional regulatory mechanisms in ferritin gene expression.
- Macromolecular Synthesis in Cells Infected by Frog Virus 3 XII. Viral Regulatory Proteins in Transcriptional and Post-Transcriptional Controls
- Post-transcriptional regulation of pro-inflammatory gene expression