Preparation of 14C-labeled Tuberculin Purified Protein Derivative
AUTOR(ES)
Landi, S.
RESUMO
14C-labeled tuberculin purified protein derivative (14C-tuberculin PPD) has been prepared from culture filtrates of Mycobacterium tuberculosis var. hominis grown in a culture medium containing uniformly labeled 14C-amino acids. With a mixture of 14C-amino acids (an acid hydrolysate of 14C-Chlorella protein) in the medium, the recoveries of 14C in the final product were higher than with 14C-labeled-l-glutamic acid. 14C-tuberculin PPD was separated into tuberculoprotein and nucleic acid by paper electrophoresis. The specific radioactivity of tuberculoprotein was substantially greater than that of the nucleic acid. 14C-tuberculin PPD is advocated as a means to measure the adsorption of tuberculin to glass or other surfaces. It could also prove useful as a means to study the structure and mode of action of tuberculin.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=547078Documentos Relacionados
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