Preparation of Cell Walls and Protoplasm of Neisseria with the Ribi Cell Fractionator

AUTOR(ES)

Martin, John E.

RESUMO

The varied pressures required for disruption of Neisseria gonorrhoeae and other species of Neisseria when the Sorvall-Ribi refrigerated cell fractionator is used in the preparation of cell walls and cellular protoplasm are reported. Optimal disruption pressure for the gonococcus was considerably less than that required for other members of the genus Neisseria. Pressures varied from 8,000 psi for N. gonorrhoeae F62, colony type 4, to 22,000 psi for the nonpathogenic Neisseria—N. sicca, N. flava, and N. catarrhalis. Representative electron photomicrographs are shown.

Documentos Relacionados

• II. HOST-REACTIVE PROPERTIES OF CELL WALLS AND PROTOPLASM FROM MYCOBACTERIA
• Preparation and chemical composition of the cell walls of Streptococcus mutans.
• MYCOBACTERIAL CELL WALLS I. : Methods of Preparation and Treatment with Various Chemicals
• IMPROVED TECHNIQUE FOR THE PREPARATION OF STREPTOCOCCAL CELL WALLS
• PREPARATION AND PROPERTIES OF CELL WALLS OF THE AGENT OF MENINGOPNEUMONITIS12