Preparation of prereduced anaerobically sterilized media and their use in cultivation of anaerobic bacteria.
AUTOR(ES)
Chan, E C
RESUMO
Several modifications of the roll-tube method have made it simpler for routine use in the isolation and growth of anaerobic bacteria. These include use of a check valve for the production of prereduced anaerobically sterilized media; a Salvarsan tube under oxygen-free gas pressure for the dispensing of molten prereduced anaerobically sterilized agar medium; a Kelly infusion bottle with a graduated pipette side arm (also under gas pressure) for quantitative delivery of fluid prereduced anaerobically sterilized media; and screw-capped prescription bottles for the cultivation of anaerobes. Colonies of Bacteroides melaninogenicus were easily identified and counted by this method. Other anaerobic bacteria have also been cultivated successfully.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=275165Documentos Relacionados
- Improved dispenser for use in preparing prereduced, anaerobically sterilized medium.
- Butyl Rubber Stoppers Increase the Shelf Life of Prereduced, Anaerobically Sterilized Media
- Evaluation of a prereduced anaerobically sterilized medium (PRAS II) system for identification anaerobic microorganisms.
- Bacteremia After Tooth Extractions Studied with the Aid of Prereduced Anaerobically Sterilized Culture Media
- Bacteremia After Tooth Extractions Studied with the Aid of Prereduced Anaerobically Sterilized Culture Media