Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies

AUTOR(ES)

Brazil, Beatriz Gomes, Lino-Neto, José, Brazil, Reginaldo Peçanha

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2003-03

RESUMO

Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5ºC) and embedded in hydroxyethyl metacrylate. Two-three µm sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue.

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