Preparative separation of flavonoids from the medicinal plant Davilla elliptica St. Hill. by high-speed counter-current chromatography

AUTOR(ES)

Rinaldo, Daniel, Silva, Marcelo Aparecido, Rodrigues, Clenilson Martins, Calvo, Tamara Regina, Sannomiya, Miriam, Santos, Lourdes Campaner dos, Vilegas, Wagner, Kushima, Hélio, Hiruma-Lima, Clélia Akiko, Brito, Alba Regina Monteiro de Souza

FONTE

Química Nova

DATA DE PUBLICAÇÃO

2006-10

RESUMO

High-speed counter-current chromatography (HSCCC) is a major tool for the fast separation of natural products from plants. It was used for the preparative isolation of the flavonoid monoglucosides present in the aerial parts of the Davilla elliptica St. Hill. (Dilleniaceae). This species is used in Brazilian folk medicine for the treatment of gastric disorders. The optimum solvent system used was composed of a mixture of ethyl acetate-n-propanol-water (140:8:80, v/v/v) and led to a successful separation of quercetin-3-O-alpha-L-rhamnopyranoside and myricetin-3-O-alpha-L-rhamnopyranoside in approximately 3.0 hours with purity higher than 95%. Identification was performed by ¹H NMR, 13C NMR and HPLC-UV-DAD analyses.

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