Preparative separation of polyphenols from artichoke by polyamide column chromatography and high-speed counter-current chromatography

AUTOR(ES)

Shu, Xikai, Wang, Mei, Liu, Daicheng, Wang, Daijie, Lin, Xiaojing, Liu, Jianhua, Wang, Xiao, Huang, Luqi

FONTE

Quím. Nova

DATA DE PUBLICAÇÃO

2013

RESUMO

An efficient method for the rapid separation and purification of polyphenols from artichoke by polyamide column chromatography in combination with high-speed counter-current chromatography (HSCCC) was successfully built. The crude ethanol extracts from dry artichoke were first pre-separated by polyamide column chromatography and divided in two parts as sample 1 and sample 2. Then, the samples were further separated by HSCCC and yielded 7.8 mg of chlorogenic acid (compound I), 24.5 mg of luteolin-7-O-β-D-rutinoside (compound II), 18.4 mg of luteolin-7-O-β-D-glucoside (compound III), and 33.4 mg of cynarin (compound IV) with purity levels of 92.0%, 98.2%, 98.5%, and 98.0%, respectively, as determined by high-performance liquid chromatography (HPLC) method. The chemical structures of these compounds were identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR).

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