Primary structure and functional organization of Drosophila 1731 retrotransposon.
AUTOR(ES)
Fourcade-Peronnet, F
RESUMO
We have determined the nucleotide sequence of the Drosophila retrotransposon 1731. 1731 is 4648 bp long and is flanked by 336 bp terminal repeats (LTRs) previously described as being reminiscent of provirus LTRs. The 1731 genome consists of two long open reading frames (ORFs 1 and 2) which slightly overlap each other. The ORF 1 and 2 present similarities with retroviral gag and pol genes respectively as shown by computer analysis. The pol gene exhibits several enzymatic activities in the following order: protease, endonuclease and reverse transcriptase. It is possible that 1731 also encompasses a ribonuclease H activity located between the endonuclease and reverse transcriptase domains. Moreover, comparison of the 1731 pol gene with the pol region of copia shows similarities extending over the protease, endonuclease and reverse transcriptase domains. We show that codon usage in the two retrotransposons is different. Finally, no ORF able to encode an env gene is detected in 1731.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=336851Documentos Relacionados
- Structural flexibility of a DNA hairpin located in the long terminal repeat of the Drosophila 1731 retrotransposon.
- A nuclear single-stranded-DNA binding factor interacts with the long terminal repeats of the 1731 Drosophila retrotransposon.
- Functional analysis of the long terminal repeats of Drosophila 1731 retrotransposon: promoter function and steroid regulation.
- Characterization and cloning of p11, a transrepressor of Drosophila melanogaster retrotransposon 1731.
- 'Site-selected' mutagenesis of a Drosophila gene using the I factor retrotransposon.