Processo reparacional em tecido cutâneo e oral de ratos submetidos à incisão cirúrgica com lasers de CO2 e diodo, e com bisturi elétrico e convencional. Uma análise morfométrica / Healing process on rat skin and toungue submitted to CO2 and diode lasers, eletrosurgery and scalpel incisions. A morphometric analysis

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

The aim of this study was to quantify and compare the healing process after incision with CO2 (2 W e 4 W) and diode lasers (2 W and 4 W), electrosurgery (2 W) and scalpel in the skin and tongue of Wistar rats (30 animals were used). One incision of each technique (6 in total) of 5 mm long and 2 mm deep was made in the dorsal skin and dorsal tongue. The animals were sacrificed at zero, 24, 48, 72 hours, 7 and 14 days after incisions. Histological sections were made from the wound areas and subjected to analyses of area of tissue damage, quantification of mast cells and density of collagen type I. The data were submitted to the ANOVA of Kruskal-Wallis and compared by Turkey-Kramer. The level of significance was set at 5% (P <0.05). The results showed difference concerning area of tissue damage, occurring significant statistically difference between the incisions with scalpel compared with those of other techniques (lasers and electrosurgery) in skin (P <0.05). On the tongue, there was statistical difference, with less tissue damage on scalpel incision compared with that of CO2 (2 W) and diode lasers (4 W) and electrosurgery incisions (P <0.05). The quantification of mast in the incisions showed statistical differences between the incisions, especially in 48 and 72h, both on the skin and tongue. Concerning the collagen quantification, statistical difference was found only in 7 th day, the collagen types I was only more evident on scalpel incision than with the electrosurgery (P <0.05). In 14 th day, the morphometric data were similar in all types of incisions. Based on these results, it is possible to conclude that at the end of the analyzed period, the morphometric data were similar in all techniques, even though there were differences concerning tissue damage, quantification of masts cell and collagen type I at the beginning of the process. Additionally, the healing process proceeds similarly in all this technique as long as the incisions are made in a similar pattern; variations may occur depending on the type of the tissue, as was shown here between the skin and tongue.

ASSUNTO(S)

rats incision in skin and tongue ratos collagen type i mast cells tissue repair colágeno tipo i reparação tecidual incisões pele e língua mastócitos

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