Produção de hidrolases pelo fungo Dicyma pulvinata, caracterização bioquímica de uma proteinase e purificação parcial desta enzima

AUTOR(ES)

Yara de Fátima Hamú

DATA DE PUBLICAÇÃO

2007

RESUMO

The fungus Dicyma pulvinata (Deuteromycotina: Hyphomycete) shows strong biocontrol potential for plant diseases, and has been found in lesions caused in plants by Microcyclus ulei (Ascomycete), the agent of the South American Leaf Blight (SALB). This disease of the rubber tree (Hevea spp.), is a limiting factor for latex production and for expansion culture in Brazil and other countries of Latin America. The biological control of M. ulei by D. pulvinata, is currently considered as promising, because it colonizes strums of phytopathogen, preventing their sporulation and, consequently, reducing defoliation and inoculum rate reinfection. It is well accepted that hydrolytic enzymes, mainly chitinase, b- glucan and protease produced by fungi with antagonic capability hydrolyse the cell wall components of phytopathogenic fungi. It is therefore, believed that control agents with higher capacity of micolytic enzymes production show also higher capacity of biological control. So, variants of D. pulvinata isolates CEN 93 (CG 774) and CEN 62 (CG 683) showing distinct capacity of M. ulei control were evaluated for their capacity to produce hydrolases. In addition, proteases produced by these two isolates were partially purified and characterized. The enzyme levels present in the culture filtrates indicated that the isolate CEN 62 had higher capacity of proteolytic enzymes production. The optima pH and temperature for each protease were about 8.0, and 25C - 50C, respectively. Ion exchange chromatographies of proteolytic enzyme samples from both isolates resulted in the separation of several protein peaks. Poliacrylamide gel electrophoresis indicated that all the protein fractions having proteolytic activity were partially purified only.

ASSUNTO(S)

protease mal-das-folhas enzimas hidrolases ciencias da saude dicyma pulvinata

Documentos Relacionados

• Produção de hidrolases pelo fungo Dicyma pulvinata, e purificação de uma beta glucanase do isolado CEN62
• Variabilidade genética e fenotípica de linhagem de Dicyma pulvinata, mantida em diferentes condições de armazenamento.
• Avaliação de pesticidas sobre o crescimento e esporulação de Dicyma pulvinata, agente de biocontrole de Microcyclus ulei.
• PRODUÇÃO, PURIFICAÇÃO PARCIAL E CARACTERIZAÇÃO BIOQUÍMICA DE GLUCOAMILASE DE Aspergillus niger OBTIDA POR FERMENTAÇÃO EM ESTADO SÓLIDO
• Caracterização molecular de isolados brasileiros de Dicyma pulvinata