Production of agaro- and carra-oligosaccharides by partial acid hydrolysis of galactans
AUTOR(ES)
Ducatti, Diogo R. B., Colodi, Franciely G., Gonçalves, Alan G., Duarte, M. Eugênia R., Noseda, Miguel D.
FONTE
Revista Brasileira de Farmacognosia
DATA DE PUBLICAÇÃO
13/05/2011
RESUMO
Agaro- and carra-oligosaccharides were produced by partial acid hydrolysis of commercial agarose and kappa-carrageenan. Di- and tetrasaccharides were purified by gel filtration chromatography and characterized by NMR (1D and 2D) spectroscopy and ESIMS. The following oligosaccharides were obtained: agarobiose, agarotetraose, kappa-carrabiose and kappa-carratetraose. Agarobiose and agarotetraose were used as standards to develop a high performance size exclusion chromatography (HPSEC) method which was utilized to study the hydrolysis rate of agarose and oligosaccharide production. Six hours of hydrolysis (0.1 M TFA, 65 ºC) produced mainly di- and tetrasaccharides. The methodology for oligosaccharide production and evaluation developed in the present work shows good potential for the production of bioactive oligosaccharides.
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