Production of antibody against T-2 toxin.

AUTOR(ES)

Chu, F S

RESUMO

Antibody against T-2 toxin was obtained after immunization of rabbits with bovine serum albumin-T-2 hemisuccinate conjugate. The antibody had greatest binding efficiency for T-2 toxin, less efficiency for HT-2, and least for T-2 triol. Cross-reaction of antibody with neosolaniol, T-2 tetraol, and 8-acetyl-neosolaniol was very weak. Diacetoxyscirpenol, trichodermin, vomitoxin, and verrucarin A essentially gave no cross-reaction with the antibody. The sensitivity of the binding assay for T-2 toxin detection was in the range of 1 to 20 ng per assay. Detailed methods for the preparation of the conjugate and the production of immune serum and methods for antibody determination are described.

Documentos Relacionados

• T-2 metabolites in the excreta of broiler chickens administered 3H-labeled T-2 toxin.
• Survey of sensitivity of twelve yeast genera toward T-2 toxin.
• Production and characterization of antibodies against HT-2 toxin and T-2 tetraol tetraacetate.
• New process for T-2 toxin production.
• Improved method for production of antibodies against T-2 toxin and diacetoxyscirpenol in rabbits.