Production of cells and viruses in a new multiple-tube tissue culture propagator.
AUTOR(ES)
Corbeil, M
RESUMO
A novel method for large-scale culture of anchorage-dependent cells is described. Culture vessels were constructed by using roller bottles and Pyrex tubing. Parallel glass tubes, equally spaced by silicone rings and inserted into the roller bottles, provided an increased culture area. The roller bottles were fitted with a second opening for medium recirculation. Culture vessels of 1,000-, 4,000-, 8,000- and 16,000-cm2 growth area were constructed to propagate Vero, BS-C-1, primary chicken embryo, and IMH-P cells. These cultured cells were used to efficiently produce several viruses: rubella virus, measles virus, poliovirus, cytomegalovirus, and herpesvirus of turkey.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=273100Documentos Relacionados
- Comparison of Membrane Filtration and Multiple-Tube Fermentation by the Colilert and Enterolert Methods for Detection of Waterborne Coliform Bacteria, Escherichia coli, and Enterococci Used in Drinking and Bathing Water Quality Monitoring in Southern Sweden
- MIXED CULTURE CYTOPATHOGENICITY: A NEW TEST FOR GROWTH OF MURINE LEUKEMIA VIRUSES IN TISSUE CULTURE
- Trypsin Action on the Growth of Sendai Virus in Tissue Culture Cells III. Structural Difference of Sendai Viruses Grown in Eggs and Tissue Culture Cells
- A New Synthetic RNA-Dependent DNA Polymerase from Human Tissue Culture Cells
- DNA replication of histone gene repeats in Drosophila melanogaster tissue culture cells: multiple initiation sites and replication pause sites.