Production of fructooligosaccharides from sugarcane molasses by Saccharomyces cerevisiae and Kluveromyces marxianus cells / Produção enzimática de frutooligossacarídeos (FOS) por leveduras a partir de melaço de cana-de-açúcar

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

Fructooligosaccharides (FOS), sugars unconventional, are oligosaccharides of naturally occurring mainly in products of plant origin. The FOS are produced through an enzymatic reaction of transfructosylation of sucrose resulting in 1-kestose (GF2), nistose (GF3) and fructofuranosyl nistose (GF4). Aiming to produce fructooligosaccharides (FOS) from sugar cane molasses, strains of yeast Saccharomyces cerevisiae and Kluveromyces marxianus were grown in medium containing sucrose or sugar cane molasses. After different periods of cultivation, aliquots were removed to analyze reducing sugars (AR) and reducing total sugars (ART) by the method of DNS (3.5- dinitrossalicilic acid), the cell growth by turbidimetry to 600nm and cell viability by staining with methylene blue. The production of fructooligosaccharides was accompanied by chromatography on paper and liquid chromatography. Through qualitative analysis on chromatography on paper was observed the presence of FOS when the medium containing molasses cultivated by Kluveromyces marxianus otherwise cultivation by Saccharomyces cerevisiae did not resulted in the production of the FOS. Both of the yeast did not show any activity for the production of FOS when the medium contained sucrose.

ASSUNTO(S)

bioquímica celular açúcares not conventional sugar cana-de-açúcar yeas melaço. sugarcane molasses. leveduras bioquímica microbiana compostos orgânicos fructooligosaccharides

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