Production of human papillomavirus type 16 virions in a keratinocyte cell line.
AUTOR(ES)
Sterling, J
RESUMO
Human papillomavirus type 16 (HPV-16) is strongly associated with carcinoma of the cervix, but the complete life cycle of the virus cannot be studied because no experimental system is available in which HPV-16 progeny are produced, and there is currently no source of HPV-16 virus particles. Most cell lines that harbor HPV-16 DNA contain the viral genome as integrated or concatenated DNA in which open reading frames are disrupted or deleted, but a human cervical keratinocyte cell line has been described which maintains HPV-16 DNA in monomeric episomal form (M.A. Stanley, H.M. Brown, M.W. Appleby, and A.C. Minson, Int. J. Cancer 43:672-676, 1989). This cell line was induced to form a stratified differentiating epithelium by grafting onto nude mice. Long-term grafts displayed the histological features of a low-grade cervical dysplasia, and terminally differentiated cells contained amplified levels of HPV-16 DNA, virus capsid antigen, and virus particles. This experimental system appears to permit the completion of the HPV-16 life cycle in virus-containing keratinocytes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=248810Documentos Relacionados
- Presence of catenated human papillomavirus type 16 episomes in a cervical carcinoma cell line.
- Human papillomavirus type 16 E5 protein affects cell-cell communication in an epithelial cell line.
- Infectious Virions Produced from a Human Papillomavirus Type 18/16 Genomic DNA Chimera
- Production of infectious duck hepatitis B virus in a human hepatoma cell line.
- Production of erythroid-potentiating activity by a human T-lymphoblast cell line.
