PRODUCTION OF PROTOPLASTS IN AN OSMOTIC MUTANT OF NEUROSPORA CRASSA WITHOUT ADDED ENZYME

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Hamilton, James G. (Tulane University School of Medicine, New Orleans, La.), and Janet Calvet. Production of protoplasts in an osmotic mutant of Neurospora crassa without added enzyme. J. Bacteriol. 88:1084–1086. 1964.—An osmotic mutant of Neurospora crassa was grown in minimal medium containing increasing concentrations of sorbose. Protoplasts were produced in the higher concentrations by inhibition of cell-wall synthesis. Similar molar concentrations of fructose, glucose, and sucrose also produced protoplasts, suggesting a relationship between inhibition of cell-wall synthesis and osmotic pressure. Increased sugar concentration led to decreased growth.

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