Production of Rosamicin: Improvement of Synthetic Medium
AUTOR(ES)
Kwak, J. W.
RESUMO
Rosamicin is one of the important macrolide antibiotics that has clinical efficacy and broad-spectrum antibacterial activity. Using a mutant strain of Micromonospora rosaria (NRRL 3718), a chemically defined medium was developed, and some fermentation conditions that are important to rosamicin biosynthesis were optimized to achieve rosamicin productivity of 230 μg/ml. Soluble starch and l-asparagine were found to be the best carbon and nitrogen sources, and a stimulative effect of magnesium and zinc ions was also found. The medium developed contains: soluble starch, 4%; l-asparagine, 0.15%; K2HPO4, 0.075%; CaCO3, 0.6%; MgSO4 · 7H2O, 0.05%; FeSO4 · 7H2O, 10−4 M; CuSO4 · 5H2O, 10−5 M; ZnSO4 · 7H2O, 10−5 M; and MnSO4 · (4-6)H2O, 10−6 M. The required air supply was about 40 mmol of O2 liter−1 · h−1 · atm−1, and the favorable culture temperature was 28 to 29°C.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=242286Documentos Relacionados
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