Production of Vi monoclonal antibodies and their application as diagnostic reagents.
AUTOR(ES)
Tsang, R S
RESUMO
Serum antibodies to Vi antigen were detected in mice immunized with the purified antigen but not with Vi-bearing Salmonella typhi whole cells. Fusion of the spleen cells from one of the Vi antibody-producing mice with NSI myeloma cells produced four stable hybridomas that secreted antibodies to Vi. Monoclonal antibodies from these four clones were all of the immunoglobulin G class and, as determined by competition, appeared to have the same epitope specificity. Despite their immunoglobulin G nature, mouse ascitic fluids induced by one of the hybridomas strongly agglutinated the Vi-positive strains of S. typhi, S. dublin, and Citrobacter strain 5396/38. Thus, 10 clinical isolates of S. typhi but not 98 strains of other bacteria were reactive in slide agglutination tests with the monoclonal antibodies.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265979Documentos Relacionados
- Characterization of murine monoclonal antibodies against serogroup B salmonellae and application as serotyping reagents.
- Direct detection of Neisseria gonorrhoeae with monoclonal antibodies characterized by serotyping reagents.
- Production of polyclonal and monoclonal antibodies against group A, B, and C capsular polysaccharides of Neisseria meningitidis and preparation of latex reagents.
- Azidopolynucleotides as photoaffinity reagents.
- Serological classification of Neisseria gonorrhoeae with monoclonal antibody coagglutination reagents.
