Proenkephalin mRNA in rat heart.
AUTOR(ES)
Howells, R D
RESUMO
The distribution of preproenkephalin mRNA in rat tissues was investigated using a homologous cDNA probe for detection. The heart was found to contain larger amounts of the mRNA than any other tissue including brain, which heretofore had been considered the richest source. The identity of the message in heart was verified by hybridizing RNA blots with a synthetic oligodeoxynucleotide that recognizes a different region of the preproenkephalin mRNA sequence than does the cDNA probe. The preproenkephalin mRNA extracted from both heart and brain contained approximately equal to 1500 bases. Dissection of heart revealed that essentially all of the message is contained within the ventricles. In contrast to the large amounts of preproenkephalin mRNA in rat heart, the opioid peptide contents is only 3% of the amount in brain. The rat heart may be a useful model for the investigation of translation control of protein synthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=323204Documentos Relacionados
- Developmental regulation of mRNA in mouse heart.
- Characterization of voltage-dependent calcium channels expressed in Xenopus oocytes injected with mRNA from rat heart.
- Electrical stimulation in vivo increases the expression of proenkephalin mRNA and decreases the expression of prodynorphin mRNA in rat hippocampal granule cells.
- Reflex splanchnic nerve stimulation increases levels of proenkephalin A mRNA and proenkephalin A-related peptides in the rat adrenal medulla.
- Cellular localization of proenkephalin mRNA in rat brain: gene expression in the caudate-putamen and cerebellar cortex.
