Promoter sequences required for transcription of Xenopus laevis histone genes in injected frog oocyte nuclei.
AUTOR(ES)
Heindl, L M
RESUMO
Amphibian oogenesis is accompanied by the accumulation of histone mRNA and proteins in the absence of ongoing DNA replication. To begin an analysis of the mechanisms by which histone gene expression is regulated during frog oogenesis and embryogenesis, we used oocyte injection to examine the upstream sequences required for transcription of genes encoding each of the five histone classes. We found that sequences necessary for maximal levels of transcription are located 100 to 200 base pairs upstream of the corresponding start sites. In this region, each promoter examined contains conserved sequence elements, several of which seem to be histone gene class specific, in addition to other, more common sequence elements believed to be used by general transcription factors.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=365423Documentos Relacionados
- Involvement of single-stranded tails in homologous recombination of DNA injected into Xenopus laevis oocyte nuclei.
- A pre-export U1 snRNP in Xenopus laevis oocyte nuclei.
- Transcription patterns of amplified Dytiscus genes coding for ribosomal RNA after injection into Xenopus oocyte nuclei.
- An oocyte-expressed alpha-tubulin gene in Xenopus laevis; sequences required for the initiation of transcription.
- 3'-end-dependent formation of U6 small nuclear ribonucleoprotein particles in Xenopus laevis oocyte nuclei.
