Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1
AUTOR(ES)
Petukhova, Galina
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase. Physical interaction between Rdh54 and Rad51 is functionally important because Rdh54 does not enhance the recombinase activity of the Escherichia coli RecA protein. Robust dsDNA-activated ATPase activity in Rdh54 generates unconstrained negative and positive supercoils in DNA. Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein–protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=316899Documentos Relacionados
- Tid1/Rdh54 promotes colocalization of Rad51 and Dmc1 during meiotic recombination
- Stimulation of D-loop formation by polypurine/polypyrimidine sequences
- RAD51-Dependent Break-Induced Replication in Yeast
- Human Rad54B is a double-stranded DNA-dependent ATPase and has biochemical properties different from its structural homolog in yeast, Tid1/Rdh54
- Recognizing the D-loop of transfer RNAs
