Properties of Malolactic Activity Purified from Leuconostoc oenos ML34 by Affinity Chromatography
AUTOR(ES)
Spettoli, Paolo
RESUMO
Malolactic activity from Leuconostoc oenos ML34 is tightly associated with lactic dehydrogenase. A simple and fast procedure, involving affinity chromatography on agarose-hexane-NAD (Agnad), was used to separate malolactic activity from lactic dehydrogenase and other proteins. The yield was ca. 86%, the purification was 5.2-fold, and the Km values for l-malate, NAD+ and Mn2+ were 2.8, 0.13, and 0.028 mM, respectively, at a pH optimum of 5.8.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=241643Documentos Relacionados
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