Protection of cultured renal tubular epithelial cells from anoxic cell swelling and cell death.

AUTOR(ES)

Kreisberg, J I

RESUMO

In order to study the relationship between cell swelling and cell death due to ischemia, we have developed an in vitro model by using primary cultures of renal tubular epithelial cells. With this model, we have studied two components of ischemia--namely, anoxia along with substrate deprivation. After 2 hr of anoxia in the absence of substrate, the cultured cells swelled and blebbed. Cells similarly treated in the presence of 8% polyethylene glycol, an oncotic agent, did not swell and bleb, and when cells were counted 18 hr later, similar numbers of cells were seen as in the untreated cultures. However, tubule cells exposed to anoxia without 8% polyethylene glycol had 50% fewer cells 18 hr later. Therefore, if cell swelling is prevented during 2 hr of anoxia, cell viability is improved.

Documentos Relacionados

• Heat-preconditioning confers protection from Ca2+-mediated cell toxicity in renal tubular epithelial cells (BSC-1)
• Endonuclease-induced DNA damage and cell death in oxidant injury to renal tubular epithelial cells.
• Inhibition of biliverdin reductase increases ANG II-dependent superoxide levels in cultured renal tubular epithelial cells
• Pyelonephritogenic Escherichia coli and killing of cultured human renal proximal tubular epithelial cells: role of hemolysin in some strains.
• Intracellular pH during "chemical hypoxia" in cultured rat hepatocytes. Protection by intracellular acidosis against the onset of cell death.